Vol. 4,No. 9, September 2014
Author(s): Mahsa Sharifat, Saeed Shirali, Padideh Ebadi
Abstract: Diabetes is a chronic disease that is associated with glycation of proteins. Hyperglycaemia in diabetes can cause hypertension, renal damage and the formation of advanced glycation end products (AGEs). The aim of this study is investigate the correlation between AGEs formation with changes of FBS, HbA1c and renal marker, β2-microglobulin in patients with type 2 diabetes and normal men. This case-controlled study was performed on 120 patients (60 men with type 2 diabetes and 60 healthy men). In this study, glucose levels were spectrophotometerically measured by the glucose oxidase method. AGEs were estimated fluorimetrically (350 / 440 nm). Serum β2-microglobulin was determined by ELISA. HbA1c is measured by the ion exchange chromatography. The results were analysed using the SPSS software version 21 and the data were reported as Mean ± SD. In this study, serum β2-microglobulin, AGEs, FBS and HbA1clevels in the diabetic group were significantly higher than in the normal group (P<0.01). Collectively, our results suggest that AGE level assay, along with determination of routine parameters, can be used as a marker for renal function estimation in diabetic patients.
International Association for Academians is an International institute with regional headquarters in Canada.