Abstract: Background and Aim: Andropause in men refers to a condition similar to menopause in women which is associated with decreased level of testosterone and Leydig cells. The results of research have shown that there is still low level of awareness and attitudes towards Andropause among health professionals. Therefore, this study aimed at assessing the level of knowledge, attitudes and performance of Larestan health care personnel regarding Andropause in 2010.Materials and methods: This study is descriptive - analytic. Sampling was done by census method. Data gathering tool was a researcher-made questionnaire for assessing knowledge, attitude and practice of nursing and medical staff regarding Andropause. Using SPSS 16. Descriptive statistics and t-test were used for data analysis. Results: The mean scores of knowledge, attitudes and practice in relation to Andropause among nursing staff were significantly lower in contrast with practitioners. Comparison of the mean scores for knowledge and practice in the two groups was statistically significant difference (P <0.001). But there was not a statistically significant difference the attitude scores (P =0.84).Conclusions: Given the importance of men's health, lack of knowledge, attitude and stated practice about Andropause among members of the health care teams, it seems essential in teaching about it in academic programs.